The First Minister unveiled the document in Glasgow, and said again that she'd launch another drive to break up Britain in the summer.
This is despite previously promising a vote on independence would be a "once in a generation" occurrence.
The SNP leader made it clear she wanted to secure a referendum re-run in the next five years.
Scottish Conservative leader Ruth Davidson said the statement would merely put Scotland through the uncertainty it previously experienced during 2014's referendum.
It also a highlighted why it was so crucial for the SNP to have a capable opposition, she said, and reinforced the fact that the SNP has breaking up Britain at the top of its agenda.
Scottish Conservative leader Ruth Davidson said:
"Nicola Sturgeon had the chance today to set a course that leaves the division of the referendum behind us.
"Instead she has made it clear she wants to put Scotland through five more years of rancour over the constitution.
"She promised the last referendum would be 'once in a generation'.
"Today, she has broken her word to the Scottish people.
"Just as bad, rather than seek a mandate in her manifesto, she says she wants the right to call a referendum on independence just as soon as she thinks she can win one.
"It is a recipe for more uncertainty which will damage jobs and weaken the economy.
"As a strong opposition, we will fight every day to demand that a future SNP government focuses on the job in hand.
"We deserve a better government, not a re-run of the referendum."